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Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), eFluor 660, eBioscience
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Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), eFluor 660, eBioscience

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인간 IFN-γ를 특이적으로 인식하는 4S.B3 단클론 항체로, eFluor 660 형광체로 표지되어 있습니다. Flow cytometry 및 intracellular staining에 최적화되어 있으며, 5 µL/test로 사용 가능합니다. 항바이러스 및 면역조절 연구용에 적합합니다.

카탈로그번호
50-7319-42
판매단위
pk
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마지막 업데이트 2025. 08. 04. 오후 11:47
Thermo Fisher Scientific 50-7319-42 IFN gamma Monoclonal Antibody (4S.B3), eFluor 660, eBioscience 100 tests pk판매 단위 pk ·
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Thermo Fisher Scientific · Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), eFluor 660, eBioscience

Applications and Tested Dilution

Application Tested Dilution Publications
Immunohistochemistry (IHC) - View 1 publication
Flow Cytometry (Flow) 5 µL (0.06 µg)/test View 71 publications
ELISA - View 1 publication

Product Specifications

항목 내용
Species Reactivity Human
Published Species Human, Mouse
Host / Isotype Mouse / IgG1, kappa
Recommended Isotype Control Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 660, eBioscience™
Class Monoclonal
Type Antibody
Clone 4S.B3
Conjugate eFluor™ 660
Excitation/Emission Max 651 / 668 nm
Form Liquid
Concentration 5 µL/test
Purification Affinity chromatography
Storage Buffer PBS, pH 7.2, with 0.2% BSA
Contains 0.09% sodium azide
Storage Conditions 4°C, store in dark, DO NOT FREEZE
Shipping Conditions Ambient (domestic); Wet ice (international)
RRID AB_11151146

Available Formats


Product Specific Information

Description:
The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN gamma). Human IFN gamma is a 17 kDa factor produced by activated T and NK cells and functions as an antiviral and antiparasitic cytokine. It acts synergistically with other cytokines such as TNF alpha to inhibit cell proliferation and modulate immune responses.

Applications Reported:
Reported for use in flow cytometric analysis and intracellular staining followed by flow cytometric analysis.

Applications Tested:
Pre-titrated and validated for intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. Recommended usage: 5 µL (0.06 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorophore Information:
eFluor® 660 is a replacement for Alexa Fluor® 647. It emits at 659 nm and is excited by the red laser (633 nm).

  • Excitation: 633–647 nm
  • Emission: 668 nm
  • Laser: Red Laser
  • Filtration: 0.2 µm post-manufacturing filtered

Target Information

IFN gamma (Interferon gamma, Type II interferon)
A macrophage activation factor produced primarily by T-lymphocytes and NK cells in response to antigens and cytokines. It is a dimeric glycoprotein (~45 kDa) composed of two 146 amino acid subunits. Functions include:

  • Antiviral and tumor antiproliferative activity
  • Induction of MHC class I and II expression
  • Macrophage activation
  • Enhancement of immunoglobulin secretion by B cells

IFN gamma acts through IFN gamma receptor I/II and the JAK-STAT pathway. It is species-specific and does not cross-react between human and mouse. Gene mutations are associated with aplastic anemia.


For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.


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