Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), eFluor 660, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 496
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 650
• eFluor 450
• eFluor 506
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

Description:
The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN gamma). Human IFN gamma is a 17 kDa factor produced by activated T and NK cells and functions as an antiviral and antiparasitic cytokine. It acts synergistically with other cytokines such as TNF alpha to inhibit cell proliferation and modulate immune responses.

Applications Reported:
Reported for use in flow cytometric analysis and intracellular staining followed by flow cytometric analysis.

Applications Tested:
Pre-titrated and validated for intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. Recommended usage: 5 µL (0.06 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorophore Information:
eFluor® 660 is a replacement for Alexa Fluor® 647. It emits at 659 nm and is excited by the red laser (633 nm).

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

IFN gamma (Interferon gamma, Type II interferon)
A macrophage activation factor produced primarily by T-lymphocytes and NK cells in response to antigens and cytokines. It is a dimeric glycoprotein (~45 kDa) composed of two 146 amino acid subunits. Functions include:

• Antiviral and tumor antiproliferative activity
• Induction of MHC class I and II expression
• Macrophage activation
• Enhancement of immunoglobulin secretion by B cells

IFN gamma acts through IFN gamma receptor I/II and the JAK-STAT pathway. It is species-specific and does not cross-react between human and mouse. Gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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