Thermo Fisher Scientific ITGB1 Recombinant Rabbit Monoclonal Antibody (32A10)
Thermo Fisher Scientific의 ITGB1 Recombinant Rabbit Monoclonal Antibody (32A10)은 인간 ITGB1 단백질을 표적으로 하는 고순도 재조합 항체입니다. Western blot, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합하며, HEK293 세포에서 발현된 Rabbit IgG 형식으로 제공됩니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 32A10 |
| Immunogen | A synthesized peptide derived from human ITGB1 (Integrin beta 1, CD29, UniProt ID: P05556-1, antigen range: 675–692) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 150 mM NaCl |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2898201 |
Product Specific Information
This antibody has been tested in direct ELISA.
Target Information
ITGB1 (Integrin Subunit Beta 1, beta1 integrin subunit, GPIIa, CD29) is a 110 kDa cell surface glycoprotein widely expressed by various cells including all leukocytes. It forms non-covalently linked heterodimers with at least six different alpha chains (α1–α6, CDa–f), determining the binding properties of beta1 (VLA) integrins.
ITGB1 acts as a cell adhesion molecule on platelets and as a component of protein complexes binding to extracellular matrix proteins. Decreased ITGB1 expression correlates with multidrug resistance in tumor cells.
It is upregulated in leukocytes during migration and plays a critical role in immune cell trafficking from blood to tissue. ITGB1 also regulates tissue damage and disease symptoms in inflammatory bowel disease.
Through ITGB1-dependent mechanisms, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1β.
Two major forms of integrin beta1 exist: beta1A and beta1D, differing by 13 amino acids. Beta1A is present in all tissues except cardiac and skeletal muscle, which express beta1D.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지: MA5-38286_Integrin_beta_1_CD29_P05556-1_Rabbit.svg)
(이미지: MA5-38286_Integrin_beta_1_CD29_P05556-1_Rabbit_PDP.jpeg)
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