Thermo Fisher Scientific PUMA alpha Polyclonal Antibody
Thermo Fisher Scientific의 PUMA alpha Polyclonal Antibody는 인간 PUMA 단백질을 인식하는 토끼 IgG 폴리클로날 항체로, WB, IHC, IP에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 고농도 액상 형태로 제공됩니다. p53 관련 세포사멸 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 130–193 of PUMA (Uniprot ID#Q9BXH1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552107 |
Product Specific Information
- Recommended positive controls: K562, THP-1, HCT116 control 0hr, HCT116 treated 30µM cisplatin 24hr
- IHC notes: Requires antigen retrieval using heat-mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PUMA is a pro-apoptotic Bcl-2 family member, along with Bax and Noxa, which are transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins, PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may directly mediate p53-induced apoptosis. Antisense inhibition of PUMA expression reduces the apoptotic response to p53, suggesting PUMA’s role in p53-mediated cell death via the cytochrome c/Apaf-1-dependent pathway.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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