Thermo Fisher Scientific PUMA alpha Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Recommended positive controls: K562, THP-1, HCT116 control 0hr, HCT116 treated 30µM cisplatin 24hr
• IHC notes: Requires antigen retrieval using heat-mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

PUMA is a pro-apoptotic Bcl-2 family member, along with Bax and Noxa, which are transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins, PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may directly mediate p53-induced apoptosis. Antisense inhibition of PUMA expression reduces the apoptotic response to p53, suggesting PUMA’s role in p53-mediated cell death via the cytochrome c/Apaf-1-dependent pathway.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.