Thermo Fisher Scientific SERCA1 ATPase Monoclonal Antibody (IIH11)
SERCA1 ATPase 단백질을 검출하는 마우스 단클론 항체로, WB, IHC, ICC/IF 등 다양한 응용에 적합합니다. Dog, Human, Mouse 등 여러 종에서 반응하며, 비결합형 액상 형태로 제공됩니다. -20°C 보관, 연구용 전용 제품입니다.
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Thermo Fisher Scientific SERCA1 ATPase Monoclonal Antibody (IIH11)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:5,000 | View 60 publications |
| Immunohistochemistry (IHC) | – | View 16 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:200 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:20–1:200 | – |
| Immunocytochemistry (ICC/IF) | 1:500 | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Guinea pig, Human, Mouse, Rabbit, Rat |
| Published Species | Dog, Human, Invertebrate, Mouse, Rabbit, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IIH11 |
| Immunogen | Purified rabbit skeletal muscle sarcoplasmic reticulum |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | PBS, ascites |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_325494 |
Product Specific Information
MA3-911 detects sarcoplasmic or endoplasmic reticulum calcium 1 (SERCA1) ATPase from canine, human, rabbit, rat, mouse, and guinea pig tissues.
It has been successfully used in Western blot, immunohistochemistry, and immunofluorescence procedures.
By Western blot, this antibody detects an ~110 kDa protein representing SERCA1 ATPase in canine skeletal muscle extracts.
Immunofluorescence staining of SERCA1 ATPase in canine skeletal muscle with MA3-911 results in strong labeling of the entire type II (fast) myofiber.
The MA3-911 antigen is purified rabbit skeletal muscle sarcoplasmic reticulum.
This antibody recognizes an epitope between amino acids 199–505 of rabbit skeletal muscle ATPase, a region that is not exposed in native sarcoplasmic reticulum.
Target Information
ATP-dependent calcium pumps maintain low cytoplasmic free calcium concentrations.
SERCA ATPases, located in intracellular organelles, are encoded by a family of structurally related enzymes.
- SERCA1: Expressed exclusively in type II (fast) skeletal muscle
- SERCA2: Generates SERCA2a (muscle-specific) and SERCA2b (ubiquitous) isoforms
- SERCA3: Found in non-muscle cells, less characterized
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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