Thermo Fisher Scientific SERCA1 ATPase Monoclonal Antibody (IIH11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

View 60 publications 60 publications

Immunohistochemistry (IHC)

-

View 16 publications 16 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunohistochemistry (Frozen) (IHC (F))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:500

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Guinea pig, Human, Mouse, Rabbit, Rat

Published species

Dog, Human, Invertebrate, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

IIH11

Immunogen

Purified rabbit skeletal muscle sarcoplasmic reticulum. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SERCA1 ATPase,uniProtId:P04191-1,ncbiNodeId:9986,antigenRange:1-1001,antigenLength:1001,antigenImageFileName:MA3-911_SERCA1_ATPase_P04191-1_House_mouse.svg,antigenImageFileNamePDP:MA3-911_SERCA1_ATPase_P04191-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS, ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325494

Product Specific Information

MA3-911 detects sarcoplasmic or endoplasmic reticulum calcium 1 (SERCA1) ATPase from canine, human, rabbit, rat, mouse and guinea pig tissues.

MA3-911 has been successfully used in Western blot, immunohistochemistry, and immunofluorescence procedures. By Western blot, this antibody detects an ~110 kDa protein representing SERCA1 ATPase in canine skeletal muscle extracts. Immunofluorescence staining of SERCA1 ATPase in canine skeletal muscle with MA3-911 results in strong labeling of the entire type II (fast) myofiber.

The MA3-911 antigen is purified rabbit skeletal muscle sarcoplasmic reticulum. This antibody recognizes an epitope between amino acids 199-505 of rabbit skeletal muscle ATPase, a region that is not exposed in native sarcoplasmic reticulum.

Target Information

ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.