Thermo Fisher Scientific GFP Monoclonal Antibody (9F9.F9), Rhodamine

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• AP
• Biotin
• DyLight 405 / 488 / 549 / 649 / 680 / 800
• FITC
• HRP
• Custom conjugation available

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot and freeze at -20°C or below.
• Avoid repeated freeze/thaw cycles.
• Centrifuge if solution is not clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.
• Immunoelectrophoresis shows a single precipitin arc against anti-Mouse Serum.
• Reactivity confirmed against recombinant GFP (Aequorea victoria) by Western blot.
• No cross-reactivity observed with RFP.

Target Information

The jellyfish Aequorea victoria produces green fluorescent protein (GFP), a 27 kDa monomeric protein that emits green fluorescence (excitation 395 nm, emission 508 nm). GFP is widely used as a reporter and fusion tag in both prokaryotic and eukaryotic systems to:

• Monitor gene expression and protein localization
• Study protein movement and subcellular dynamics
• Assess protein–protein interactions (e.g., yeast two-hybrid system)
• Measure distances between proteins via FRET

GFP fluorescence is stable, species-independent, and suitable for numerous live-cell imaging applications.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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