Thermo Fisher Scientific Phospho-ER (Ser167) Polyclonal Antibody
인간 ER(Ser167) 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC(P, PFA)에서 사용 가능. 항원 친화 크로마토그래피로 정제되었으며 0.5 mg/mL 농도의 액상 형태. MCF-7 세포에서 양성 대조로 사용되며 핵 내 위치 확인 가능.
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Thermo Fisher Scientific Phospho-ER (Ser167) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 2–10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide surrounding the epitope -LASTN- with phosphorylation site Ser167 of human estrogen receptor |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 25 mM Tris with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: MCF-7
- Cellular location: Nucleus
Target Information
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor that, upon binding to estrogen, undergoes a conformational change enabling dimerization and DNA binding to estrogen response elements (ERE). ER regulates gene transcription via coactivator or corepressor recruitment.
Two forms exist—ER alpha and ER beta—encoded by ESR1 and ESR2. Alternative splicing produces multiple ER-alpha isoforms (66 kDa, 53 kDa, 47 kDa, 35 kDa). ERs are expressed in various tissues, essential for sexual development and reproduction, and implicated in breast cancer, endometrial cancer, and osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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