Thermo Fisher Scientific Phospho-Histone H3 (Ser10) Monoclonal Antibody (GT921)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GT921

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Histone H3S10ph (phospho Ser10). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Histone H3,uniProtId:P68431-1,ncbiNodeId:9606,antigenRange:10,antigenLength:136,antigenImageFileName:MA5-31502_Histone_H3_P68431-1_House_mouse.svg,antigenImageFileNamePDP:MA5-31502_Histone_H3_P68431-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:Histone H3,uniProtId:Q71DI3-1,ncbiNodeId:9606,antigenRange:10,antigenLength:136,antigenImageFileName:MA5-31502_Histone_H3_Q71DI3-1_House_mouse.svg,antigenImageFileNamePDP:MA5-31502_Histone_H3_Q71DI3-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2787133

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (100%), Chimpanzee (100%).

Positive Control: Nocodazole treated HeLa cell (24hr).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.