Thermo Fisher Scientific Phospho-Histone H3 (Ser10) Monoclonal Antibody (GT921)
Phospho-Histone H3 (Ser10) 단일클론 항체로 인간, 마우스, 랫트 시료에 반응. Western blot, IHC, ICC/IF에 사용 가능. 고순도 Protein G 정제, PBS 기반 액상 형태. 세포주기 연구 및 히스톤 인산화 분석에 적합.
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Thermo Fisher Scientific Phospho-Histone H3 (Ser10) Monoclonal Antibody (GT921)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT921 |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human Histone H3S10ph (phospho Ser10) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787133 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Chimpanzee (100%).
- Positive Control: Nocodazole treated HeLa cell (24 hr).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome.
Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translational modifications of histones, such as methylation, acetylation, and phosphorylation, influence chromatin structure and gene expression.
Phosphorylation of Histone H3 at Ser10 is associated with chromosome condensation during mitosis and meiosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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