Thermo Fisher Scientific CCL28 Polyclonal Antibody, PeproTech
Human CCL28 단백질을 인식하는 Rabbit Polyclonal 항체로, Western Blot 및 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 500-P297-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CCL28 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human MEC (CCL28) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2930284 |
Product Specific Information
AA Sequence of recombinant protein:
SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MEC (CCL28). Anti-Human MEC (CCL28)-specific antibody was purified by affinity chromatography employing an immobilized Human MEC (CCL28) matrix.
Sandwich ELISA:
To detect Human MEC (CCL28) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MEC (CCL28) (500-P297Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human MEC (CCL28).
Western Blot:
To detect hMEC by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hMEC is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland, and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T-cells, and eosinophils. MEC contains six cysteines, including the four highly conserved cysteine residues present in CC chemokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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