Thermo Fisher Scientific PRRX1 Monoclonal Antibody (OTI1C2), TrueMAB
PRRX1 단백질을 인식하는 Mouse IgG2a 단일클론 항체. Western blot 및 IHC(P)에서 검증됨. 무보존제, 동결건조 형태로 제공되며 -20°C 보관. 연구용으로 전사 조절 관련 단백질 분석에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C2 |
| Immunogen | Full length human recombinant protein of human PRRX1 produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The homeobox DNA-binding domain is a 60 amino acid motif conserved among species, functioning in transcriptional regulation via a helix-turn-helix structure.
PRRX1 (paired related homeobox 1) is a 245 amino acid nuclear protein containing one OAR domain and one homeobox DNA-binding domain, belonging to the paired homeobox family.
It acts as a transcriptional co-activator enhancing serum response factor (SRF) activity, mediating SRF-dependent gene expression in growth and differentiation.
Additionally, PRRX1 regulates creatine kinase-M transcription, contributing to mesodermal muscle type formation.
PRRX1 exists as two alternatively spliced isoforms, PMX1-A and PMX1-B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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