Thermo Fisher Scientific HNRNPM Monoclonal Antibody (OTI1E12), TrueMAB
Human HNRNPM 단백질을 인식하는 Mouse IgG1 단일클론 항체. Western blot 및 IHC(P) 검증 완료. 무보존제, 동결건조 형태로 제공되며 재구성 후 1 mg/mL 농도로 사용 가능. RNA 결합 단백질 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E12 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–261 of human HNRNPM produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Heterogeneous nuclear ribonucleoproteins (hnRNPs) bind nascent RNA polymerase II transcripts and are involved in transcript-specific packaging and alternative splicing of pre-mRNAs.
The M1–M4 hnRNP proteins form a cluster of four abundant pre-mRNA binding proteins that interact with poly(G) and poly(U) RNA homopolymers.
They belong to the ribonucleoprotein consensus sequence family and contain a methionine/arginine-rich hexapeptide repeat motif (MR repeat), similar to that found in the 64 kDa subunit of cleavage stimulation factor involved in 3'-end maturation of pre-mRNAs.
Homologous proteins exist across diverse eukaryotes from humans to yeast.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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