Thermo Fisher Scientific Phospho-CDK4 (Thr172) Recombinant Superclonal Antibody (9HCLC)
CDK4 Thr172 인산화 형태를 검출하는 재조합 Superclonal 항체로 높은 특이성과 감도를 제공합니다. 인간에 반응하며, 단일 클론의 일관성으로 로트 간 변동성이 적습니다. ICC/IF 실험에 적합하며 단백질 A로 정제되어 안정적입니다.
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Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: 2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Antibody |
| Clone | 9HCLC |
| Immunogen | Peptide corresponding to Human CDK6 (174–180) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2632990 |
Product Specific Information
- Predicted to react with Monkey, Pig, and Horse.
- Recombinant rabbit Superclonal™ antibodies are composed of multiple recombinant monoclonal antibodies, combining polyclonal sensitivity with monoclonal specificity.
- Each lot maintains consistent composition, eliminating variability typical of polyclonal antibody production.
- Formerly called “Recombinant Polyclonal Antibody”, now rebranded as “Recombinant Superclonal™ Antibody”.
- Physical product and performance remain unchanged.
Target Information
CDK4/cyclin D3 serine/threonine kinase is a dimeric complex involved in controlling cell proliferation during the G1 phase of the cell cycle.
CDK4 forms a complex with D-type cyclins and is inhibited by p16. It mediates phosphorylation of the C-terminal region of Rb protein, leading to transcriptional repression of the E2F complex.
CDC37 and HSP90 associate with CDK4 not bound to D-type cyclins.
SMAD3 is a major substrate of CDK4 and CDK2.
CDK6 and CDK4 phosphorylate Rb protein at different residues (CDK6: Thr821, CDK4: Thr826).
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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