Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), NovaFluor Violet 800, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.6 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 661, 737
• Brilliant Violet 421
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 510, 530, 610-70S, 660-120S, 725, 800
• NovaFluor UV 765
• NovaFluor Violet 690
• NovaFluor Yellow 810
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

Description:
The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes and most peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation, proliferation, or apoptosis depending on assay conditions. It serves as a phenotypic marker for TCR beta-expressing T cells.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse thymocytes and splenocytes. Recommended use ≤ 0.6 µg/test in 100 µL final volume. Cell number can range from 10⁵ to 10⁸ cells/test. Titrate antibody for optimal performance.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL per sample or per 100 µL of cell sample (10³–10⁸ cells) for single-color controls.
• Add buffer to antibody cocktails before mixing with cells.

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered nucleic acid dye structures, minimizing spillover and spectral spread.

• Excitation: 408 nm
• Emission: 800 nm
• Laser: 488 nm (Blue)

Target Information

T cell receptors (TCRs) discriminate foreign from self-peptides presented by MHC class II molecules, crucial for adaptive immunity. Self-peptide recognition is linked to autoimmune diseases, while mutated self-peptides are associated with tumors. Superantigen engagement can cause toxic shock syndrome. Autoantibodies to TCR V beta segments have been found in rheumatoid arthritis and systemic lupus erythematosus, potentially modulating immune responses.

Human T cells primarily express TCR alpha-beta with CD4 or CD8. Double-negative (DN) alpha-beta T cells are increased in certain autoimmune and immunodeficiency disorders. Gamma-delta T cells, found mainly in epithelia, exhibit limited TCR diversity and unique antigen recognition, contributing to antitumor and immunoregulatory functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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