Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), NovaFluor Blue 725, eBioscience

Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), NovaFluor Blue 725, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: ≤ 0.6 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 700
• APC, APC-eFluor 780
• Biotin, Brilliant UV 496, 661, 737
• Brilliant Violet 421
• eFluor 450, 506
• FITC, Functional Grade
• NovaFluor Blue 510, 530, 610-70S, 660-120S, 800
• NovaFluor UV 765
• NovaFluor Violet 690, 800
• NovaFluor Yellow 810
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes and most peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation, proliferation, or apoptosis depending on assay conditions. It serves as a phenotypic marker for TCR beta-expressing T cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse thymocytes and splenocytes (≤0.6 µg/test)
• Each product includes 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.
• Compatibility Note: NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01): Required for optimal staining. Add 5 µL per sample to antibody cocktails or single-color controls.
• Technology: NovaFluor conjugates are based on Phiton™ technology, enabling engineered fluorescent signatures optimized for minimal spillover and spread.
• Excitation: 493 nm; Emission: 720 nm; Laser: 488 nm (Blue)

Target Information

T cell receptors (TCRs) are essential for recognizing peptides presented by MHC class II molecules, enabling adaptive immune responses.

• Recognition of self-peptides is associated with autoimmune diseases.
• Mutant self-peptides may relate to tumor development.
• Superantigen engagement of TCRs can cause toxic shock syndrome.
• Autoantibodies to V beta segments are found in RA and SLE patients, potentially modulating inflammatory responses.
• Most human T cells express TCR alpha-beta with CD4 or CD8; gamma-delta T cells show distinct antigen recognition and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures.

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