Thermo Fisher Scientific Phospho-A-Raf (Ser299) Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:100

Product Specifications

Target Information

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1.
Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity, broadly expressed in nearly all cell lines tested to date. It is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus.
The unregulated kinase activity of v-Raf has been associated with transformation and mitogenesis, while Raf-1 activity is normally suppressed by a regulatory N-terminal domain.
A-Raf, another member of the Raf gene family, shows substantial homology to Raf-1 within the kinase domain but less elsewhere. Expression of A-Raf is highest in urogenital tissues and kidney, and lowest in brain tissue.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.