Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), Alexa Fluor 488, eBioscience
Alexa Fluor 488로 결합된 IL-17A 단일클론 항체로, 마우스 및 랫트 IL-17A를 특이적으로 인식합니다. Flow cytometry, IHC, ICC 등 다양한 응용에 적합하며, 0.5 µg/test로 최적화되어 있습니다. 염증성 사이토카인 연구 및 Th17 세포 분석에 이상적입니다.
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Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), Alexa Fluor 488
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 5 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | 1 |
| Immunocytochemistry (ICC/IF) | - | 1 |
| Flow Cytometry (Flow) | 0.5 µg/test | 91 |
| ELISA | - | 3 |
| Functional Assay | - | 1 |
| Miscellaneous PubMed | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio17B7 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_763579 |
Additional Conjugate Formats
- APC
- Biotin
- Brilliant UV 395 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 650 / 711 / 786
- eFluor 450 / 506 / 660
- FITC
- PE / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Custom conjugation available
Product Specific Information
The eBio17B7 antibody reacts with mouse and rat IL-17A without recognizing IL-17F. IL-17A is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in autoimmune and inflammatory diseases such as rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
Applications Reported: Suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested: Verified by intracellular staining and flow cytometric analysis of restimulated, Th17-polarized mouse splenocytes using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00). Recommended use ≤ 0.5 µg per test (100 µL final volume). Cell concentration may vary from 10⁵ to 10⁸ cells/test. Titrate for optimal performance.
Compatible with Foxp3 buffer system (Product # 00-5523-00).
- Excitation: 488 nm
- Emission: 519 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a 32 kDa disulfide-linked homodimer cytokine produced by CD4+ T cells. It promotes inflammatory responses and induces secretion of IL-6, IL-8, G-CSF, and PGE2 in epithelial and fibroblastic cells. IL-17A regulates NF-kappa B and MAPK activities, enhances COX-2 and nitric oxide production, and supports hematopoietic progenitor proliferation and neutrophil maturation.
Usage
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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