Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), Alexa Fluor 488

Applications and Tested Dilution

Product Specifications

Additional Conjugate Formats

• APC
• Biotin
• Brilliant UV 395 / 615 / 661 / 737 / 805
• Brilliant Violet 421 / 650 / 711 / 786
• eFluor 450 / 506 / 660
• FITC
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Custom conjugation available

Product Specific Information

The eBio17B7 antibody reacts with mouse and rat IL-17A without recognizing IL-17F. IL-17A is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in autoimmune and inflammatory diseases such as rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.

Applications Reported: Suitable for intracellular staining followed by flow cytometric analysis.

Applications Tested: Verified by intracellular staining and flow cytometric analysis of restimulated, Th17-polarized mouse splenocytes using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00). Recommended use ≤ 0.5 µg per test (100 µL final volume). Cell concentration may vary from 10⁵ to 10⁸ cells/test. Titrate for optimal performance.

Compatible with Foxp3 buffer system (Product # 00-5523-00).

• Excitation: 488 nm
• Emission: 519 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a 32 kDa disulfide-linked homodimer cytokine produced by CD4+ T cells. It promotes inflammatory responses and induces secretion of IL-6, IL-8, G-CSF, and PGE2 in epithelial and fibroblastic cells. IL-17A regulates NF-kappa B and MAPK activities, enhances COX-2 and nitric oxide production, and supports hematopoietic progenitor proliferation and neutrophil maturation.

Usage

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

제품 이미지