Thermo Fisher Scientific EGFR Polyclonal Antibody
EGFR 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, IP 등 다양한 분석에 사용 가능. 인간 EGFR의 1100–1150 잔기 영역을 면역원으로 제작. 액상 형태, 1 mg/mL 농도, 4°C 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:50,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 1–10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 1100 and 1150 of human Epidermal Growth Factor Receptor |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Monkey
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that mediates signaling in response to various growth factors. Overexpression has been linked to numerous types of cancer. EGFR is encoded by the EGFR gene located on chromosome 7 in humans and belongs to the HER/ErbB family, which includes ERbB2, ERbB3, and ERbB4. Binding of ligands such as EGF and TGF-α to the extracellular domain induces receptor dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and in squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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