Thermo Fisher Scientific EGFR Polyclonal Antibody
EGFR 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, IP 등에 적합. Human 시료 반응성 확인. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 제공. 4°C 보관, 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:50,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 1–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 1100 and 1150 of human Epidermal Growth Factor Receptor |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Monkey
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that mediates signaling in response to various growth factors. Overexpression of EGFR is associated with multiple cancer types and is targeted by both biological and small-molecule therapeutics. The EGFR gene is located on chromosome 7 in humans and belongs to the HER/ERbB family of proteins, which includes ERbB2, ERbB3, and ERbB4. EGFR activation involves ligand binding (e.g., EGF, TGF-α), receptor dimerization, and autophosphorylation of tyrosine residues in the cytoplasmic domain. Overexpression has been observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and in squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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