
Thermo Fisher Scientific CD57 Monoclonal Antibody (TB01 (TBO1)), eFluor 660, eBioscience
인간 CD57(HNK-1, Leu-7)을 인식하는 TB01 단클론 항체로, eFluor 660 형광체가 결합된 Flow Cytometry용 시약입니다. NK 및 NKT 세포 분석에 적합하며, 5 µL/test로 사용됩니다. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 5 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgM |
| Recommended Isotype Control | Mouse IgM Isotype Control (11E10), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TB01 (TBO1) |
| Conjugate | eFluor™ 660 |
| Excitation/Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2574162 |
Available Formats
- Unconjugated
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- Request custom conjugation
Product Specific Information
Description:
This TB01 monoclonal antibody reacts with human CD57 (also known as HNK-1 and Leu-7), a 110-kDa cell surface glycoprotein expressed on a subset of natural killer (NK) cells and NK T cells.
Applications Reported:
This TB01 (TBO1) antibody has been reported for use in flow cytometric analysis.
Applications Tested:
This TB01 (TBO1) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
Recommended usage: 5 µL (0.25 µg) per test.
A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL.
Cell number should be determined empirically (10⁵–10⁸ cells/test).
Fluorophore Information:
eFluor® 660 is a replacement for Alexa Fluor® 647.
It emits at 659 nm and is excited with a red laser (633 nm).
Ensure the instrument can detect this fluorochrome.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD57, also known as HNK-1 and Leu-7, is an oligosaccharide antigenic determinant present on various polypeptides, lipids, and chondroitin sulfate proteoglycans.
Its function is not fully understood. CD57 is expressed on subsets of NK and NKT cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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