Thermo Fisher Scientific ISG15 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Target Information

Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or as a free protein. It is secreted from monocytes in response to type I interferons and promotes natural killer (NK) cell proliferation and non-MHC-restricted cytotoxicity. Its synthesis is stimulated by IFN-α, IFN-β, or IFN-ω, but not IFN-γ. ISG15 expression can also be induced by overexpression of interferon regulatory factors or influenza B virus.

ISG15 is secreted by monocyte, T-lymphocyte, B-lymphocyte, fibroblast, and epithelial cell lines. It enhances NK cell proliferation, augments cytotoxicity, and induces IFN-γ from T cells, suggesting its role in amplifying immunomodulatory effects of interferons. Intracellularly, ISG15 functions as a ubiquitin homolog. Mutations in the ISG15 gene can result in Immunodeficiency 38 with basal ganglia calcification (IMD38).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.