Thermo Fisher Scientific APG12 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

ELISA

• Tested Dilution: 1:1,000–1:5,000

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot and freeze at -20°C or below. Avoid freeze/thaw cycles.
• Centrifuge if not clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.
• Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Target Information

ATG12 is a member of the autophagy protein family and forms a conjugate with ATG5, exhibiting ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy.
This conjugate associates with innate immune response proteins such as RIG-I and VISA (IPS-1), inhibiting type I interferon production and allowing viral replication.
ATG12 interacts with ATG10 in human embryonic kidney cells in the presence of ATG7.
At least two isoforms of ATG12 are known. Autophagy is essential for cellular protein turnover and nutrient preservation under starvation, and may be defective in tumor cells.
This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.