Thermo Fisher Scientific Phospho-GRK2 (Ser670) Recombinant Rabbit Monoclonal Antibody (HL1035)

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

Store as concentrated solution. Centrifuge briefly prior to opening vial.
For short-term storage (1–2 weeks), store at 4°C.
For long-term storage, aliquot and store at -20°C or below.
Avoid multiple freeze-thaw cycles.

Target Information

ADRBK1 (GRK2) is a serine/threonine kinase. Many G protein-coupled receptors (GPCRs) are phosphorylated by G protein-dependent receptor kinases (GRKs).
GRK2 (83 kDa), one of six identified members, is ubiquitously expressed in mammals.
After ligand binding and interaction with heterotrimeric G proteins, GPCRs are phosphorylated by GRKs.
Internalization of GPCRs, regulated by beta-arrestin-1, activates the Ras–Raf–ERK1&2 signaling pathway.
GRK2 activity is tightly regulated by phosphorylation (PKC, Src, ERK1&2) and protein interactions.
ERK phosphorylates and inactivates GRK2 on serine 670 in a negative feedback mechanism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.