Thermo Fisher Scientific Phospho-MCM4 (Ser54) Polyclonal Antibody
Phospho-MCM4 (Ser54) 폴리클로날 항체로, 인간·마우스·랫 시료에서 MCM4 단백질의 인산화 상태를 검출. Western blot 및 IHC(P) 적용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체로, 90 kDa 단백질 검출에 적합. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human MCM4 around the phosphorylation site of Serine 54 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2553785 |
Product Specific Information
- Detects endogenous protein at a molecular weight of approximately 90 kDa.
- Purity >95% by SDS-PAGE.
Target Information
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) essential for the initiation of eukaryotic genome replication.
The hexameric MCM complex is a key component of the pre-replication complex (pre_RC) and is involved in replication fork formation and recruitment of other DNA replication-related proteins.
The MCM complex (including MCM2, 6, and 7) possesses DNA helicase activity and acts as a DNA unwinding enzyme.
Phosphorylation of this protein by CDC2 kinase reduces helicase activity and chromatin binding.
This gene is located on chromosome 8, adjacent to PRKDC/DNA-PK, involved in DNA double-strand break repair.
Alternatively spliced transcript variants encoding the same protein have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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