Thermo Fisher Scientific PUM1 Recombinant Rabbit Monoclonal Antibody (JG37-75)
PUM1 단백질을 표적으로 하는 재조합 토끼 단클론 항체로, WB, IHC, ICC/IF, Flow Cytometry 등 다양한 응용 가능. 높은 특이성과 재현성을 제공하며, HEK293 세포에서 발현된 IgG 형 항체. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JG37-75 |
| Immunogen | Recombinant protein within Human Pumilio 1 aa 79–223 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2899017 |
Product Specific Information
This product is predicted to work in Immunoprecipitation at a dilution of 1:10–1:50.
Target Information
PUM1 (pumilio homolog 1) is a sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. It belongs to the PUF family of evolutionarily conserved RNA-binding proteins. PUM1 binds to the RNA consensus sequence known as the Pumilio Response Element (PRE, 5'-UGUANAUA-3'), related to the Nanos Response Element (NRE). PUM1 mediates post-transcriptional repression by recruiting the CCR4-POP2-NOT deadenylase complex, resulting in translational inhibition and mRNA degradation. Mutations in the gene can cause spinocerebellar ataxia 47.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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