Thermo Fisher Scientific BAF53A Polyclonal Antibody
Thermo Fisher Scientific의 BAF53A Polyclonal Antibody는 인간 및 생쥐 시료에서 BAF53A 단백질을 검출하기 위한 고품질 항체입니다. Western blot, IHC, IP 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제된 액상 형태 제품입니다. 연구용으로만 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:100–1:500
Immunoprecipitation (IP)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 25 and 75 of human BRG1-associated factor, 53 kDa subunit A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
BAF53A is a member of the actin-related protein (ARP) family, sharing significant sequence identity with conventional actins. Both actins and ARPs possess an ATP-binding cleft known as the actin fold. ARPs participate in cellular processes such as vesicular transport, spindle orientation, nuclear migration, and chromatin remodeling.
This gene encodes a 53 kDa subunit of the BAF (BRG1/brm-associated factor) complex in mammals, functionally related to the SWI/SNF complex in yeast and Drosophila. The complex facilitates transcriptional activation by counteracting chromatin-mediated repression. Together with beta-actin, BAF53A is essential for maximal ATPase activity of BRG1 and association of the BAF complex with chromatin/matrix.
Three transcript variants encoding two different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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