Thermo Fisher Scientific BAK1 Monoclonal Antibody (4C2)
BAK1 단백질을 인식하는 Thermo Fisher Scientific의 Mouse IgG2b 단일클론 항체. Western blot, IHC, ICC/IF, Flow cytometry, ELISA 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 동결건조 형태로 장기 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
| ELISA | – |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4C2 |
| Immunogen | E.coli-derived human BAK/BAK1 recombinant protein (Position: A22–S211). Human BAK shares 78.3% amino acid sequence identity with mouse BAK. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2884059 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators involved in various cellular activities. This protein localizes to mitochondria and induces apoptosis by interacting with and accelerating the opening of the mitochondrial voltage-dependent anion channel, leading to loss of membrane potential and release of cytochrome c. It also interacts with tumor suppressor P53 after exposure to cell stress.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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