Thermo Fisher Scientific ATG5 Polyclonal Antibody
ATG5 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS 버퍼에 0.02% sodium azide 포함. 자가포식 및 세포사멸 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:400 | - |
| Immunocytochemistry (ICC/IF) | 1:250 | - |
| Flow Cytometry (Flow) | Assay-Dependent | - |
| ELISA | Assay-Dependent | - |
| Immunoprecipitation (IP) | 1:10–1:500 | - |
| Radioimmune Assays (RIA) | Assay-Dependent | - |
| in situ PLA (PLA) | Assay-Dependent | - |
| Immunomicroscopy (IM) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Fruit fly, Fish, Guinea pig, Human, Mouse, Non-human primate, Pig, Reptile, Rat, Xenopus, Zebrafish |
| Published Species | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide made to an N-terminal region of the human ATG5 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2274520 |
Product Specific Information
- In Western Blot, a band is seen at ~56 kDa representing the ATG5–ATG12 complex. The molecular weight of human ATG5 is ~33 kDa.
- For paraffin-embedded tissues, antigen retrieval using sodium citrate buffer (pH 6.0) is recommended.
- Suggested positive controls: antigen standard for ATG5 (transient overexpression lysate), mouse wildtype ES cell lysate.
Target Information
ATG5 (Autophagy Related 5) is a key component in the autophagy pathway and plays an important role in apoptosis. It is involved in mitochondrial quality control, regulation of innate antiviral immune responses, lymphocyte development, MHC II antigen presentation, and adipocyte differentiation.
Following conjugation to ATG12, the ATG5–ATG12 complex participates in autophagosome formation and contributes to autophagic cell death via interaction with FADD. It also associates with innate immune response proteins such as RIG-I and VISA (IPS-1), modulating type I interferon production. Dysregulation of ATG5 is linked to diseases such as spinocerebellar ataxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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