Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), eFluor 450, eBioscience
Mouse CD45.2 단백질을 인식하는 모노클로날 항체로, eFluor 450 형광체가 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, 405/445 nm 파장에서 검출 가능합니다. 모든 백혈구에 발현되는 CD45.2를 표지하여 면역분석에 활용됩니다.
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Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), eFluor 450, eBioscience
Applications and Tested Dilution
- Immunohistochemistry (IHC): Not specified
View 4 publications - Flow Cytometry (Flow): 0.5 µg/test
View 62 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 104 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11039533 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 532, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV series (395–805)
- Brilliant Violet series (421–786)
- eFluor 506
- FITC
- NovaFluor Blue series (510–660)
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright series (436–780)
- Custom conjugation available upon request
Product Specific Information
The 104 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.2-expressing mouse strains such as C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometric analysis of mouse splenocytes
- Recommended ≤ 0.5 µg/test (100 µL final volume)
- Cell number range: 10⁵–10⁸ cells/test
- Antibody should be titrated for optimal performance
Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emits at 445 nm. Ensure instrument compatibility with this fluorochrome.
Laser: Violet (405 nm)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of surface proteins in lymphocytes. It plays a critical role in lymphocyte development and antigen signaling by regulating Src-family kinases.
CD45.2 exists as multiple isoforms due to alternative splicing, differing in extracellular domains but sharing transmembrane and cytoplasmic regions. These isoforms vary by cell type and physiological state, influencing membrane localization and signaling.
Additionally, CD45.2 modulates integrin-mediated signal transduction for cell survival and participates in DNA fragmentation during apoptosis. CD45RA is one isoform with restricted expression among lymphoid cell subtypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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