Thermo Fisher Scientific SBP2 Polyclonal Antibody
Thermo Fisher Scientific SBP2 Polyclonal Antibody는 인간, 마우스, 랫트 반응성을 가지며 SECISBP2 단백질을 인식합니다. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. 고순도 친화 크로마토그래피 정제, 안정적 PBS/glycerol 저장용액 구성.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 585–854 of human SECISBP2 (NP_076982.3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.92 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2855760 |
Product Specific Information
Immunogen sequence:
DELISTPSVE DKSEEPPGTE LQRDTEASHL APNHTTFPKI HSRRFRDYCS QMLSKEVDAC VTDLLKELVR FQDRMYQKDP VKAKTKRRLV LGLREVLKHL KLKKLKCVII SPNCEKIQSK GGLDDTLHTI IDYACEQNIP FVFALNRKAL GRSLNKAVPV SVVGIFSYDG AQDQFHKMVE LTVAARQAYK TMLENVQQEL VGEPRPQAPP SLPTQGPSCP AEDGPPALKE KEEPHYIEIW KKHLEAYSGC TLELEESLEA STSQMMNLNL
Target Information
Eukaryotes require a selenocysteine (Sec) insertion sequence (SECIS) element in the 3′ untranslated region of the mRNA to decode the UGA codon as Sec.
SECIS-binding protein 2 (SBP-2) specifically binds selenoprotein mRNAs to form a functional complex and is essential for the insertion of Sec into selenoproteins.
Purified SBP-2 interacts specifically with the SECIS element in the phospholipid hydroperoxide glutathione peroxidase mRNA.
SBP-2 shows binding activity in the liver and testis as well as hepatoma cells.
SBP-2 binds to a conserved RNA binding domain shared with several ribosomal proteins and eukaryotic translation termination release factor 1.
A second domain located N-terminal to the RNA binding domain required for Sec insertion allows SBP-2 to stably associate with the ribosomal fraction of cells.
SBP-2 preferentially stimulates incorporation directed by the selenoprotein P and phospholipid hydroperoxide glutathione peroxidase SECIS elements.
SBP-2 may have a distinct function in selecting the ribosomes for Sec insertion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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