Thermo Fisher Scientific SIRPA Monoclonal Antibody (OTI1B5), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:4,000

Flow Cytometry (Flow)

• Tested Dilution: Assay-Dependent

Product Specifications

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
To use this carrier-free antibody for conjugation experiments, perform an additional desalting step.
Recommended desalting product: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882)

Target Information

SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on myeloid cells such as granulocytes, dendritic cells, macrophages, mast cells, and hematopoietic stem cells.
It acts as a substrate for activated tyrosine kinases (EGFR, PDGFR, src, insulin receptor) and negatively regulates receptor tyrosine kinase-coupled signaling pathways.

Binding of SIRP alpha to CD47 triggers phosphorylation of ITIM motifs in its cytoplasmic domain, recruiting tyrosine phosphatases SHP-1 and SHP-2.
This interaction regulates processes such as macrophage-mediated phagocytosis inhibition and T cell/DC activation.
SIRP alpha influences cellular responses to serum, growth factors, insulin, oncogenes, and adhesion, playing a broad role in physiological and pathological contexts.
Cancer cells often overexpress CD47, activating SIRP alpha and preventing macrophage-mediated clearance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.