Thermo Fisher Scientific GADD45A Monoclonal Antibody (OTI2B7), TrueMAB
인간 GADD45A 단백질을 인식하는 Mouse IgG1 단일클론 항체. Western blot 검증 완료. 동결건조 형태로 제공되며, PBS buffer(8% trehalose) 포함. 세포 스트레스 반응 및 G2/M 체크포인트 연구용.
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Applications
- Western Blot (WB)
Tested Dilution: 1:4,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B7 |
| Immunogen | Full length human recombinant protein of human GADD45A produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
GADD45 alpha, beta, and gamma are evolutionarily conserved nuclear proteins acting as stress sensors for cellular and environmental damage.
They are required for activation of the G2/M checkpoint induced by UV radiation or alkylating agents.
GADD45-induced G2/M checkpoint is regulated through inactivation of Cdc2-cyclin B1 kinase.
GADD45 forms a complex with p21 and Cdc2, serving as a core for interaction with other cell cycle regulators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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