Thermo Fisher Scientific Phospho-Crk (Tyr221) Polyclonal Antibody
Crk 단백질의 Tyr221 인산화 형태를 인식하는 Rabbit Polyclonal Antibody. Western blot에 최적화되어 있으며, 인간 및 햄스터 시료 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태로 제공되며, 보존제가 포함되지 않음. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Hamster, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pTyr221 of human CrkII |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10984499 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other tyrosine-phosphorylated proteins.
Target Information
The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. It is involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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