Thermo Fisher Scientific Nhe-1 Polyclonal Antibody
Thermo Fisher Scientific의 Nhe-1 Polyclonal Antibody는 인간 Nhe-1 단백질을 인식하는 토끼 다클론 항체입니다. WB, IHC, ICC/IF에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며, 4°C 또는 -20°C에서 안정적으로 보관할 수 있습니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human Nhe-1. Recombinant protein control fragment (Product #RP-103140) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2644661 |
Product Specific Information
- Immunogen sequence:
SPESVDLVNE ELKGKVLGLS RDPAKVAEED EDDDGGIMMR SKETSSPGTD DVFTPAPSDS PSSQRIQRCL SDPGPHPEPG EGEPFFPKG - Highest antigen sequence identity: Mouse - 83%, Rat - 83%
- HIER pH 6 was used for IHC antigen retrieval.
- Cells used for ICC/IF were permeabilized and fixed with PFA/Triton™ X-100.
Target Information
The Na⁺/H⁺ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is inhibited by amiloride and activated by growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 anchors actin filaments to maintain cortical cytoskeleton integrity through interactions with ERM proteins (ezrin, radixin, moesin). It may also play a role in hypertension. At least two isoforms of Nhe-1 are known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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