Thermo Fisher Scientific RAD21 Recombinant Rabbit Monoclonal Antibody (ARC2276)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

ChIP assay (ChIP)

5 µg antibody for 10 µg-15 µg of Chromatin

-

ChIP-sequencing (ChIP-Seq)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC2276

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 532-631 of human Rad21 (O60216) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RAD21,uniProtId:O60216-1,ncbiNodeId:9606,antigenRange:532-631,antigenLength:631,antigenImageFileName:MA5-37979_RAD21_O60216-1_Rabbit.svg,antigenImageFileNamePDP:MA5-37979_RAD21_O60216-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897897

Product Specific Information

Positive Samples: HeLa, A-549, NIH/3T3, C6

Immunogen sequence: EKEDDEEEED EDASGGDQDQ EERRWNKRTQ QMLHGLQRAL AKTGAESISL LELCRNTNRK QAAAKFYSFL VLKKQQAIEL TQEEPYSDII ATPGPRFHII

Target Information

This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Alternative splicing results in multiple transcripts variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.