Thermo Fisher Scientific GPNMB Monoclonal Antibody (OTI1G10), TrueMAB
GPNMB 단백질을 인식하는 마우스 단클론 항체로, Western blot에 최적화됨. HEK293T 세포에서 발현된 인간 GPNMB 전장 단백질을 면역원으로 사용. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G10 |
| Immunogen | Full length human recombinant protein of human GPNMB produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The GPNMB gene encodes a type I transmembrane glycoprotein homologous to pMEL17, a melanocyte-specific protein.
GPNMB is expressed in lowly metastatic human melanoma cell lines and xenografts, but not in highly metastatic ones.
It may play a role in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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