
Thermo Fisher Scientific Donkey anti-Sheep IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, TRITC
Donkey에서 생산된 anti-Sheep IgG (H+L) TRITC 결합 2차 항체로, 높은 교차흡착 특성과 낮은 비특이적 결합을 제공합니다. 면역세포염색(ICC/IF)에 적합하며, 552/578 nm 형광 특성을 가집니다. 연구용으로만 사용 가능합니다.
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Applications
- Immunocytochemistry (ICC/IF): 1:500–1:20,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Sheep |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | TRITC |
| Excitation / Emission Max | 552 / 578 nm |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C. Avoid freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534726 |
| Target | IgG |
| Cross Adsorption | Against human, mouse, and rabbit IgG |
| Antibody Form | Whole Antibody |
Additional Formats
- AP
- Biotin
- FITC
- HRP
- Custom conjugation available upon request
Product Specific Information
Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.)
Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Shelf life: 1 year from date of receipt.
Prepare working dilution prior to use and discard after use.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin sheep serum proteins or IgG from human, mouse, or rabbit.
Target Information
Anti-Sheep secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins.
They are useful in detection, sorting, or purification of their specified targets.
Secondary antibodies provide:
- Increased versatility for multiple detection systems (e.g., HRP, AP, fluorescence)
- Enhanced sensitivity through signal amplification
These antibodies are typically generated by immunizing the host with pooled immunoglobulins from the target species and are further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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