Thermo Fisher Scientific GCLC Recombinant Rabbit Monoclonal Antibody (JA08-03)
GCLC 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, Flow Cytometry에 적합합니다. 높은 특이성과 민감도, 일관된 로트 간 재현성, 동물 유래 성분 무첨가 포뮬레이션을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA08-03 |
| Immunogen | Synthetic peptide within Human GCLC aa 588–637 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810111 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
Glutamate-cysteine ligase (GCLC), also known as gamma-glutamylcysteine synthetase, is the first rate-limiting enzyme in glutathione synthesis. It consists of a heavy catalytic subunit and a light regulatory subunit.
This gene encodes the catalytic subunit, while the regulatory subunit is encoded by a separate gene on chromosome 1p22-p21. Mutations in this gene are associated with hemolytic anemia due to enzyme deficiency and increased susceptibility to myocardial infarction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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