Thermo Fisher Scientific ZIC1 Recombinant Rabbit Monoclonal Antibody (JA31-60)
ZIC1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, Flow cytometry에 적합합니다. 높은 특이성과 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션으로 생산되었습니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA31-60 |
| Immunogen | Synthetic peptide within Human Zic1 aa 1–50 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810115 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed in HEK293 cells. Individual clones are screened to select the best candidates for production.
Advantages:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
The ZIC1 gene encodes a member of the ZIC family of C2H2-type zinc finger proteins, which play crucial roles during development. Aberrant expression of ZIC1 is associated with medulloblastoma, a childhood brain tumor. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 4 (ZIC4) on chromosome 3. ZIC1 acts as a transcription factor that can bind and transactivate the apolipoprotein E gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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