Thermo Fisher Scientific Cytokeratin 4 Polyclonal Antibody
Human KRT4에 특이적인 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며 PBS/glycerol 버퍼에 보관. 세포 및 조직 내 Cytokeratin 4 발현 연구에 적합.
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Thermo Fisher Scientific Cytokeratin 4 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human KRT4 (control fragment: Product #RP-95140) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.10 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2790407 |
Product Specific Information
Immunogen sequence:
GSTSTGGISG GLGSGSGFGL SSGFGSGSGS GFGFGGSVSG SSSSKIISTT TLNKRR
Target Information
Cytokeratins are a subfamily of intermediate filament proteins characterized by biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between approximately 40 kDa and 68 kDa and have isoelectric pH values between 4.9 and 7.8.
Human cytokeratins are numbered 1 to 20, and their expression patterns are characteristic of epithelial type and maturation level. These patterns are widely used to distinguish epithelial malignancies. Cytokeratin antibodies assist in differential tumor diagnosis using immunohistochemistry and are valuable tools in cytopathology and flow cytometry assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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