Thermo Fisher Scientific 4EBP1 Polyclonal Antibody
Thermo Fisher Scientific의 4EBP1 Polyclonal Antibody는 인간 및 설치류 시료에 반응하며, Western blot, IHC, IP 등에 적합합니다. 항원 친화 크로마토그래피로 정제된 액상 제품으로, 안정적인 저장 및 높은 특이성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Full length human 4E-BP1 recombinant protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2546423 |
Product Specific Information
- Recommended positive controls: HeLa, H1299, HCT116, NB4, HL60
- Predicted reactivity: Mouse (90%), Rat (92%), Pig (90%), Bovine (92%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5′ end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. The protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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