Thermo Fisher Scientific CD223 Monoclonal Antibody (8B12E10)
인간 CD223(LAG-3)을 인식하는 Mouse IgG1 단클론 항체로 다양한 응용(WB, IHC, Flow, ELISA)에 사용 가능. 고순도 Protein G 정제, PBS/glycerol buffer로 안정화. T세포 활성 조절 연구용에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8B12E10 |
| Immunogen | Recombinant Human Lymphocyte activation gene 3 protein (29–450AA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.03% Proclin 300 |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_3091046 |
Target Information
LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. It binds to MHC class II with higher affinity than CD4 and is involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells typically express LAG-3 at higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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