
Thermo Fisher Scientific Collagen III Polyclonal Antibody
Human 및 Mouse 반응성을 가진 Rabbit Polyclonal Collagen III 항체. WB, IHC, ICC 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제된 액상 형태로, 높은 특이성과 재현성을 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 3 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 3 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to amino acids 1247–1389 of Collagen III alpha1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.49 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552139 |
Product Specific Information
- Recommended positive controls: HeLa, SK-N-SH, SK-N-AS, mouse brain, rat brain
- Predicted reactivity: Mouse (92%), Rat (92%), Pig (93%), Chicken (86%), Rhesus Monkey (97%), Bovine (93%)
- IHC notes: Requires antigen retrieval using heat-mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Collagen III is a fibrillar collagen found in extensible connective tissues such as skin, lung, and the vascular system, often associated with type I Collagen. Mutations in this gene are linked to Ehlers-Danlos syndrome type IV and aortic or arterial aneurysms. Although alternate transcripts have been detected, they result from mutations affecting splicing, often leading to exon exclusion.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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