Thermo Fisher Scientific Calreticulin Polyclonal Antibody
Calreticulin 단백질에 특이적인 Rabbit Polyclonal 항체로, WB, IHC, ICC, IP 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성 확인. 항원 친화 크로마토그래피로 정제된 액상 항체로, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–214 of Calreticulin (Uniprot ID: P27797) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552138 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, A375, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2
- Predicted reactivity: Mouse (94%), Rat (94%), Zebrafish (82%), Xenopus laevis (83%), Pig (94%), Rabbit (95%), Rhesus Monkey (100%), Bovine (95%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Calreticulin (Calr) is a calcium-binding chaperone that promotes folding, oligomeric assembly, and quality control in the endoplasmic reticulum (ER) through the calreticulin/calnexin cycle. Calr, a lectin, transiently interacts with monoglucosylated glycoproteins synthesized in the ER. It also interacts with the DNA-binding domain of NR3C1 to mediate nuclear export and may play a role in maternal gene expression regulation and oocyte maturation via calcium homeostasis regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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