Thermo Fisher Scientific MAPK15 Polyclonal Antibody, FITC
MAPK15 단백질을 인식하는 FITC 결합 토끼 폴리클로날 항체로, Western blot, ELISA, IP에 사용 가능. 인간, 마우스, 랫트 반응성. 액상 형태로 -20°C 암소 보관. 세포 신호전달 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 500–544 on human Mitogen-activated protein kinase 15 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The ERKs are a subfamily of the MAPKs involved in cell growth and differentiation. Extracellular signal-regulated kinase 8 (Erk8) is a large MAP kinase regulated by serum and the c-Src non-receptor tyrosine kinase. ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5 and can negatively regulate transcriptional co-activation of androgen receptor and GRalpha by Hic-5 in a kinase-independent manner. This suggests a broader role for ERK8 in the regulation of nuclear receptors beyond estrogen receptor alpha. Erk8 is also a novel effector of RET/PTC3, contributing to RET biological functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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