Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), eFluor 660, eBioscience
Ki-67 단백질을 인식하는 SolA15 단클론 항체로, 세포 증식 마커 분석에 사용됩니다. eFluor 660 형광 표지로 유세포분석, 면역조직화학, 면역세포염색에 적합합니다. 마우스, 랫드, 인간 등 다양한 종에 반응하며, 4°C 어두운 곳에 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 |
| Immunohistochemistry (IHC) | Assay-Dependent | 70 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | 4 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | 42 |
| Flow Cytometry (Flow) | 0.125 µg/test | 66 |
| Functional Assay | - | 1 |
| Miscellaneous (PubMed) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Mouse, Pig, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SolA15 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574234 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 660, 700, Plus 594
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805 series
- Brilliant Violet 421–786 series
- eFluor 450, 506, 570, 615
- FITC, PE, PE-Cy5, PE-Cy7, PE-eFluor 610, PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, and mitosis), but absent in resting cells (G0).
Ki-67 serves as a proliferation marker, with the Ki-67 labeling index used to evaluate tumor growth and prognosis.
The SolA15 antibody also reacts with human, non-human primate, and canine Ki-67.
Applications include intracellular staining for flow cytometry, immunohistochemistry, and immunocytochemistry.
Tested Applications:
- Flow cytometry of stimulated mouse splenocytes (≤0.125 µg/test)
- Immunocytochemistry on fixed and permeabilized C2C12 cells (≤10 µg/mL)
Fluorophore Information:
- eFluor® 660 replaces Alexa Fluor® 647
- Excitation: 633–647 nm / Emission: 668 nm
- Laser: Red
- Post-manufacturing filtration: 0.2 µm
Target Information
Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle.
It associates with euchromatin and heterochromatin through its FHA domain and shows cell cycle–dependent localization.
Ki-67 is widely used as a cell proliferation marker, correlating with tumor grade and prognosis.
In neoplastic tissues, low Ki-67 index indicates low-grade tumors.
It is also used as a neuronal marker for cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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