Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), eFluor 660, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 660, 700, Plus 594
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805 series
• Brilliant Violet 421–786 series
• eFluor 450, 506, 570, 615
• FITC, PE, PE-Cy5, PE-Cy7, PE-eFluor 610, PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, and mitosis), but absent in resting cells (G0).
Ki-67 serves as a proliferation marker, with the Ki-67 labeling index used to evaluate tumor growth and prognosis.

The SolA15 antibody also reacts with human, non-human primate, and canine Ki-67.
Applications include intracellular staining for flow cytometry, immunohistochemistry, and immunocytochemistry.

Tested Applications:

• Flow cytometry of stimulated mouse splenocytes (≤0.125 µg/test)
• Immunocytochemistry on fixed and permeabilized C2C12 cells (≤10 µg/mL)

Fluorophore Information:

• eFluor® 660 replaces Alexa Fluor® 647
• Excitation: 633–647 nm / Emission: 668 nm
• Laser: Red
• Post-manufacturing filtration: 0.2 µm

Target Information

Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle.
It associates with euchromatin and heterochromatin through its FHA domain and shows cell cycle–dependent localization.
Ki-67 is widely used as a cell proliferation marker, correlating with tumor grade and prognosis.
In neoplastic tissues, low Ki-67 index indicates low-grade tumors.
It is also used as a neuronal marker for cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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