Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (93), PE, eBioscience

Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (Clone 93), PE, eBioscience™

Applications and Tested Dilution

• Flow Cytometry (Flow): 0.125 µg/test
• Miscellaneous PubMed (Misc): -

View 34 publications
View 1 publication

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Red 685 / 700 / 710 / 755
• NovaFluor Yellow 690
• PE-Cyanine7
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436 / 600
• Request custom conjugation

Product Specific Information

The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32.
CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are low-affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤ 0.125 µg/test.
A test is defined as the amount of antibody that stains a cell sample in 100 µL final volume (10⁵–10⁸ cells/test).
Careful titration recommended for optimal performance.

Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD16 is a 50–65 kDa cell surface molecule expressed in two forms:

• Transmembranous form: NK cells and some T cells
• Phosphatidylinositol-linked form: Granulocytes

CD16 acts as a low-affinity receptor for IgG (FcR III) and mediates antibody-dependent cellular cytotoxicity (ADCC).

CD32 is a 40 kDa glycoprotein acting as a low-affinity IgG receptor (Fc gamma RII), involved in:

• Endocytosis
• Activation of secretion
• Cytotoxicity and immunomodulation

CD32 is expressed by B cells, monocytes, granulocytes, and platelets.
It plays a key role in phagocytosis of immune complexes and regulation of antibody production by B cells.
Variations in the CD32 gene may increase susceptibility to systemic lupus erythematosus (SLE).
Multiple transcript variants encoding different isoforms of CD32 exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지