Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), PE, eBioscience
인간 CD16(FcγRIII)을 인식하는 마우스 단클론 항체로, PE 형광표지되어 유세포분석(Flow Cytometry)에 적합합니다. 친화 크로마토그래피로 정제되었으며, 4°C 암소 보관이 필요합니다. 다양한 형광체 포맷으로 제공되어 다중 분석에 활용 가능합니다.
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- 12-0168-4x (2개 옵션)
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioCB16 (CB16) |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11043436 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 480
- eFluor 450
- eFluor 506
- FITC
- Functional Grade
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Red 710
- NovaFluor Red 725
- NovaFluor Yellow 660
- NovaFluor Yellow 730
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
The eBioCB16 monoclonal antibody recognizes human CD16 (Fc gammaRIII), a low-affinity receptor for IgG with an apparent molecular weight of 50–80 kDa.
CD16 exists as two forms:
- CD16A (FcγRIIIA): Found in macrophages and NK cells (transmembrane form)
- CD16B (FcγRIIIB): Found in neutrophils (GPI-anchored form)
Two polymorphisms of CD16B (NA-1 and NA-2) are known, with NA-2 homozygotes showing lower phagocytic capacity. CD16 binds IgG immune complexes, preferentially IgG1 and IgG3, and triggers signaling cascades leading to ADCC, phagocytosis, degranulation, and proliferation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated for flow cytometry on normal human peripheral blood cells
- Recommended Use: 5 µL (0.06 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
- Excitation: 488–561 nm
- Emission: 578 nm
- Laser Compatibility: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD16 (encoded by FCGR3A) recognizes the Fc portion of IgG and mediates clearance of immune complexes, ADCC, and enhancement of viral infection.
- Expression: NK cells (membrane-bound, FCGR3A) and neutrophils (GPI-anchored, FCGR3B)
- Chromosome: 1
- Clinical Relevance: Mutations linked to immunodeficiency 20, recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia
- Variants: Multiple alternatively spliced isoforms exist
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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