Thermo Fisher Scientific Glutamate-Cysteine Ligase Regulatory Subunit Monoclonal Antibody (CPTC-GCLM-1)
Thermo Fisher Scientific의 GCLM 단일클론 항체(CPTC-GCLM-1)는 인간 GCLM 단백질을 인식하는 Mouse IgG2a 항체로, Western blot 및 IHC에 적합합니다. 단백질 A/G로 정제되었으며 PBS 버퍼에 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1–2 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CPTC-GCLM-1 |
| Immunogen | Recombinant human full-length GCLM protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Gamma-glutamylcysteine synthetase (gamma-GCS) is the rate-limiting enzyme for glutathione (L-gamma-glutamyl-L-cysteinylglycine, GSH) synthesis.
GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant.
Gamma-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants, and cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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