
Thermo Fisher Scientific Glutamate-Cysteine Ligase Regulatory Subunit Monoclonal Antibody (CPTC-GCLM-1)
인간 GCLM 단백질을 인식하는 단클론 항체로, Western blot 및 IHC(P)에서 사용 가능. 단백질 A/G로 정제된 액상 항체이며, 보존제 무첨가. -20°C 또는 -80°C 보관 권장. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CPTC-GCLM-1 |
| Immunogen | Recombinant human full-length GCLM protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage conditions | -20°C or -80°C if preferred |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed):
Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Gamma-glutamylcysteine synthetase (gamma-GCS) is the rate-limiting enzyme for glutathione (L-gamma-glutamyl-L-cysteinylglycine, GSH) synthesis.
GSH is a vital intra- and extracellular antioxidant present ubiquitously in mammalian cells.
Gamma-GCS is a heterodimer consisting of a heavy catalytic subunit and a light regulatory subunit that responds to inflammation, phenolic antioxidants, heat shock, oxidants, and cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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