Thermo Fisher Scientific RACGAP1 Monoclonal Antibody (OTI4C5), TrueMAB
RACGAP1 단백질을 인식하는 마우스 단일클론 항체로 Western blot 및 IHC(P) 실험에 적합함. 인간, 마우스, 랫트 반응성. 무보존제, 동결건조 형태로 제공되며 1 mg/mL 농도. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4C5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 123–238 of human RACGAP1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
RAC, a small GTPase in the ras family, regulates membrane ruffle, lamellipodia, and filopodia formation. It plays a key role in cytoskeletal actin organization and cellular transformation.
p21 activated kinases (PAKs) are direct targets of active Rac and Cdc42, inducing polarized cytoskeletal structures in fibroblasts.
RAC1 and RAC2 are essential components of NADPH oxidase, responsible for generating free radicals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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