Thermo Fisher Scientific RACGAP1 Monoclonal Antibody (OTI4H8), TrueMAB
RACGAP1 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI4H8). Western blot과 IHC(Paraffin)에 적합하며, Human, Mouse, Rat에 반응. 동결건조 형태로 제공되며, PBS buffer(8% trehalose) 내 보존. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4H8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 123–238 of human RACGAP1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
RAC, a small GTPase in the ras family, regulates the formation of membrane ruffles, lamellipodia, and filopodia. It is involved in cytoskeletal actin organization and transformation.
The p21 activated kinases (PAKs) are direct targets of active Rac and Cdc42, which can induce the assembly of polarized cytoskeletal structures when expressed in fibroblasts.
RAC1 and RAC2 are essential components of NADPH oxidase, the enzyme responsible for generating free radicals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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